LIPS

A label-free tool for drug-target engagement analysis

LIPS is an innovative, label-free method designed to measure drug-target interactions with high accuracy in native environments, including cultured cells and patient tissues. By leveraging the natural charge and behavioural changes in proteins upon drug binding, LIPS separates proteins into aggregated (pellet) and soluble (supernatant) phases using pH-variable plates and hydrophobic conditions. This allows precise quantification of drug-induced effects without artificial markers.

Key features:

  • Dynamic insights: Captures real-time engagement kinetics and dose-response relationships.
  • Multi-method validation by combining:
    • Immunoblotting  to assess Ph Phase shift upon drug binding
    • Label-free proteomics to identify and quantify target-proteins network
    • Surface plasmon resonance (SPR) for binding kinetics and specificity
  • Versatility: Applicable to diverse samples, from cell lines to clinical tissues.

Why it matters?

LIPS provides a holistic view of drug efficacy, speed, and specificity, enabling safer, targeted therapy development. Its label-free design preserves protein integrity, while integrated readouts ensure robust, actionable data for optimizing drug candidates and advancing precision medicine.

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